The microfluidic NanoGene assay has been developed for pathogen detection in water systems.  A magnetic bead and quantum dot nanoparticle based NanoGene assay has been proved for a sensitive gene quantification that is robust and specific in environmental samples.  In order to further develop the NanoGene assay in a portable, in-situ capable system, a microfluidic platform has been applied. As a result, the microfluidic NanoGene assay has successfully quantified picomolar concentrations of the eaeA gene of Escherichia coli O157:H7.  The ability to mix and hybridize the nanoparticles within a microfluidic chip and flow rates, residence time, temperature, concentration of reagents, and size of magnetic beads were optimized.  Parameters determined during component optimization were used to begin incorporating the microfluidic NanoGene assay into a portable, in-situ detection system.  This portable in-situ pathogenic detection system will yield faster, more accurate results because the need for sample transport will be eliminated. The microfluidic NanoGene assay can be used for the sustainable management of water systems by providing rapid, accurate, and robust in-situ detection of pathogens.  This will ensure safe, reliable waterways that do not pose threat to public health.

Development of a microfluidic NanoGene assay for the pathogen detection in water

244th National Meeting (2012)

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