Multi-isotope Imaging Mass Spectrometry (MIMS) is the combination of an ion microscope-secondary ion mass spectrometer with tracer methods and intensive quantitative image analysis. MIMS allows one to image the distribution and to measure the accumulation of molecules labelled with stable or radioactive isotopes  within  subcellular domains in volume smaller than 1micron cubed. MIMS can provide unique information in almost all fields of biomedical research, particularly in  two broad areas: 1. metabolic turnover (protein, lipids and sugars in sub-cellular domains using as precursors for example 15N-labeled amino-acids or 13C labeled fatty acids) 2. cell lineage tracking individual cells within large populations by labelingDNA. It could also be appplied to drug localization.Because stable isotopes are integral part of living organisms, they are not toxic. This allows one to label DNA with a stable isotope precursors for period of time that could reach years. This allows applications of MIMS to human.

Quantitation of cell metabolism and DNA replication at high resolution with multi-isotope imaging mass spectrometry

244th National Meeting (2012)

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