Recently, magnetic resonance (MR) reporter proteins have been developed toward in vivo protein analysis. However, practical in vivo application of MR reporter protein is prevented by the inherent low sensitivity of MR modality. Therefore, we attempted to overcome this problem using dynamic nuclear polarization, which efficiently hyperpolarize 1H, 13C and 15N nuclei to enhance the MR signal of isotope labeled probe by a factor of ~100,000. We developed a promising candidate for a hypersensitive MR reporter protein, which is comprised of mouse lactate dehydrogenase-X and a hyperpolarized pyruvic acid derivative, substrate Y (T. Nishihara et al., Chem. Sci., 2012 , 3, 800�806.). The X�Y pair shows high MR sensitivity and sufficient reaction kinetics; thus this reporter protein can be detected by monitoring the 13C NMR signal of hyperpolarized product Yr in crude biological mixtures.

Mouse lactate dehydrogenase X: A new magnetic resonance reporter protein using hyperpolarized pyruvic acid derivative

244th National Meeting (2012)

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