Metabolomics involves measurement of metabolites present within cells. In this study, NMR and mass spectrometry were utilized as metabolic profiling tools to monitor variation in metabolite levels of photosensitized prostate cancer (PCa) cells. Photosensitizers (PSs), CAS and TAS, were synthesized. Fluorescence microscopy of CAS-sensitized cells showed more apoptosis than TAS.  Necrotic cells were observed at higher concentration and prolonged light exposure. NMR spectra were collected on CAS- or TAS-incubated PCa cells exposed to 650 nm light.  CAS-sensitized apoptotic cells showed higher levels of choline, taurine, sugars and creatine, but lower levels of lactate-lipid. Metabolite levels in necrotic cells decreased due to loss of structural integrity. Mass spectral data showed presence of low MW (<500) region corresponding to metabolites responsible for physiological functions, while mass range 700-800 is observed for cells under stress. NMR and Mass spectral data of intact cancer cells can provide information on the biochemical processes of cells.

Monitoring in vitro response of photosensitized human LNCaP prostate cancer cells by NMR and mass spectrometry

244th National Meeting (2012)

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