Since PNA was shown to be mitogenic for HT29 colon cancer cells by binding to T-antigen (Gal�1-3GalNAca-Ser/Thr) highly expressed in colon cancer cells, namely to CD44v6, and stimulating c-Met phosphorylation followed by subsequent mitogen-activated protein kinase activation (Singh et al. Glycobiology 2001 & 2006), we studied effects of PNA on LS180 colon cancer cell growth. Addition of PNA, at 2.5 and 25 �g/ml, in serum-free medium containing 0.1% BSA significantly stimulated the growth of LS180 cells to levels of 118.0 � 0.05 % and 123.3 � 0.02 % (n=8), respectively, of that without PNA on day 3. HGF, the ligand for c-Met, did not stimulate LS180 cell growth. Growth stimulatory effects of PNA were inhibited by anti-Tn-antigen (GalNAca-Ser/Thr) MLS128, which suppressed LS180 cell growth in the medium containing 1% FBS (Morita et al. Biosci. Trends 2009). The results suggested possible interactions between PNA and MLS128 signaling pathways.

Effects of T-antigen specific peanut agglutinin (PNA) on LS180 colon cancer cell growth: Potential interaction with MLS128 monoclonal antibody against Tn-antigen

244th National Meeting (2012)

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