We describe a very highly sensitive and rapid immunosensor-based approach for the detection of food-borne pathogen Listeria monocytogenes (Lc) in buffer and milk as a food matrix. The electrically-excited and electrically measured PEMC sensors show resonance frequency decrease as antigen binds to sensor. After immobilizing an IgG anti-Lc on the sensor it was installed in a flow cell, and then exposed in a flow format to the spiked samples.  Successful detection up to as low a concentrations as 102 cells/mL was achieved. Secondary and tertiary antibody labeling was used for confirmation of sensor response. Sample concentrations tested were 102 to 105  cells/mL, and the sensor response was log-linear with antigen concentration in both buffer and milk.

Rapid detection of <i>Listeria monocytogenes</i> in milk using a novel piezoelectric cantilever (PEMC) sensor

244th National Meeting (2012)

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Rapid detection of Listeria monocytogenes in milk using a novel piezoelectric cantilever (PEMC) sensor