We developed an assay using headless Aedes aegypti larvae to bypass cuticular penetration.  Headless control larvae, when stimulated with a probe, show vigorous lateral contractile movement and are responsive for up to five hours.  Larvae exposed to toxicants display sluggish movement or no movement at all.  The LC50 of many of the known potassium channel blockers in this assay were high (> 100 ppm), whereas tetraethylammonium chloride and 4-aminopyridine (4-AP) had LC50 values in the low ppm range.  Force transducer measurements of electrically evoked A. aegypti muscle contraction showed that a catechol, PRC 725, increased the amplitude of evoked contractions and the baseline tension, similar to 4-AP.  In addition, an intact larval bioassay showed blackening of the larvae and 100% mortality at 100 ppm PRC725, suggesting that catechol oxidation was involved.  It was also toxic to adult Anopheles gambiae by contact.  The implications of these results for insecticide development are discussed.

Evaluation of novel insecticidal compounds as new mosquitocides for control of <i>Aedes aegypti </i>and <i>Anopheles gambiae</i>

244th National Meeting (2012)

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Evaluation of novel insecticidal compounds as new mosquitocides for control of Aedes aegypti and Anopheles gambiae