This study demonstrates a strategy for one-step formed enzyme/ferrocene/graphene three-dimensional structure successfully enables the well-studied ferrocene-based second-generation biosensors for selective continuous online in vivo measurements of neurochemicals. The strategy is based on the combination the advantage of PMS-bonded enzymes which allows for maximal retention of activity with the advantages of graphene which enhance the conductivity as well as ferrocene which was stably immobilized by the electrostatic interaction with graphene to accelerate electron-shuttling. By using glucose oxidase (GOD) as the model biorecognition units, we demonstrate the validity of the ferrocene-based second-generation biosensors for selective in vivo neurochemical measurements. We find that the biosensors developed with the strategy demonstrated in this study can well be used as the selective detector for continuous online detecting of striatum glucose of guinea pigs, by integrating with in vivo microdialysis. This study essentially a selective approach to developing electrochemical biosensors effectively for in vivo neurochemical measurements, which is envisaged to play a role on understanding the molecular basis of physiological and pathological events.

One-step formation of self-assembled enzyme/ferrocene/graphene composite film for online continuous neurochemical monitoring

244th National Meeting (2012)

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