The effect of dietary tocopherol (Toc) isomers, including a-Toc, ?-Toc and d-Toc, in modulating inflammatory response of intestinal cell lines derived from adult (Caco-2) and infant (FHs74Int) origin were determined.  Toc isomers exhibited anti-inflammatory effect by reducing IL8 secretion from Caco-2 cells challenged with interferon gamma/phorbol mirystate acetate (IFNg/PMA). An opposing trend however was observed in the infant FHs74Int cells regardless of IFNg/PMA stimulation. At the same time, differential regulation of Nf?B pathway signaling pro-inflammatory cytokines and Nrf-2 pathway signaling expression of antioxidant enzymes by Toc isomers in the two cell lines was observed.  ?-Toc was most effective in stimulating both Nf?B and Nrf-2 pathways. In addition, the transcript level of two key enzymes involved in glutathione synthesis was down-regulated by d-Toc in the infant FHs74Int cells, while no difference was observed in adult Caco-2 cells, suggesting potential differences in glutathione biosynthesis that may affect oxidative status thus IL8 expression.

Effect of dietary tocopherol isomers on inflammatory response of adult- and infant-derived intestinal cell lines

244th National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.