Chemical biomarkers are often used to identify animals that have consumed baits used to deliver pharmaceuticals to wildlife species. Iophenoxic acids (IOPAs) have been used as bait markers in a variety of wildlife species. Traditionally, quantification of IOPA was accomplished by measurement of serum iodine levels. However, this method can often be confounded by dietary intake of iodine. We investigated various extraction and clean-up approaches for direct quantification of IOPAs in serum of white-tailed deer. Performance of the direct quantification method was explored through the use of various techniques, including the Bradford protein assay, post-column infusion, and quantitation of selected phospholipids. Ion suppression and enhancement of the analytes in the MS source were taken into account as well as removal of selected phospholipids which contribute to this effect. The final method employed a solid-phase extraction clean-up with recoveries averaging 95% at serum levels from 0.25 to 100,000 ng/mL.

Tell-"tail" indicator: Quantifying iophenoxic acids in deer serum via LC/MS/MS analysis

244th National Meeting (2012)

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