Since the introduction of genetically engineered (GE) crops in the mid-1990s, immunochemistry-based protein detection methods have played an important role in various areas across the agricultural biotechnology industry. This technology is widely used in trait discovery research, product development, registration, seed production, commercialization, and numerous post-launch activities. The primary use of immunoassays is to quantify the amount of GE protein present in a product or to verify the presence or absence of GE material in a product. Lately this technology has been developed to meet high-throughput needs for handling large quantities of samples, and for analyzing growing multi-trait stack products. A good quality method should be specific, sensitive, accurate, and reproducible which requires a rigorous method validation prior to its application in GE crops. The immunoassay method validation requirement and the challenges of its applications in GE products will be presented. In addition, emerging new technologies for protein detection in agricultural biotechnology will be discussed in detail.

Immunochemistry-based protein detection in genetically engineered crops

244th National Meeting (2012)

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