Hyphenated mass spectrometry (gas chromatography/mass  spectrometry) techniques have been employed as one of the primary  analytical tools for investigating the effects of ionizing radiation,  chemical/biological carcinogens and oxygen derived free radicals on the  induction and subsequent repair of oxidatively-induced DNA damage (DNA  lesions) in living systems.  Certain DNA lesions, such as  7,8-dihydro-8-oxoguanine (8-OH-Gua), are not only established mutagens,  but have also been utilized as biomarkers of systemic oxidative stress.   The National Institute of Standards and Technology (NIST) has  established a comprehensive research program focused on identifying and  characterizing the DNA damaging mechanisms of commercially relevant  engineered engineered nanoparticles (NPs) using models of increasing  biological complexity through the application of sensitive hyphenated  mass spectrometry tools for the quantification of oxidatively-induced  DNA damage.  We present an overview of our recent findings from studies  on metal (AuNP), metal oxide (CuO NP), and carbon-based (MWCNTs)  nanomaterials.

Tools and approaches for the assessment of nanomaterial induced oxidative DNA damage

244th National Meeting (2012)

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