Engineered nanomaterial (ENM) dispersion and agglomeration has been shown to play an important role in nonmaterial toxicity. Method and medium used for dispersion, can affect dispersion stability as well as cellular uptake of ENM. Application of chemical surfactants and high-energy sonication procedures can lead to changes in ENM surface properties (surface defects), contamination, ENM fracturing or disintegration. The end toxicity results might change based on the dispersion status. Thus, leading to ambiguous observations and misinterpretation. Shape detection in solution, especially for high-aspect ratio ENM (e.g. CNTs) is challenging and not possible with existing technologies. We explored the novel Scanning Ion Occlusion Sensing (SIOS) technology, for particle-by-particle size and shape detection with different aspect ratio ENM, dispersed in complex cell culture media. The technology was found to offers some unique insights for ENM size, concentration analysis and shape detection in solution. It also has potential for studying ENM-medium interactions, aggregation and mobility of ENM in solution.

In-solution characterization of nanomaterials for in-vitro studies based on Scanning Ion Occlusion Sensing (SIOS)

244th National Meeting (2012)

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