Two-Photon-Fluorescence (TPF) has several advantages over the conventional confocal fluorescence microscopy, like better tissue penetration, reduced photo-damage and less photo-bleaching of the fluorescent molecule.  Consequently, the design of novel materials with large two-photon absorption cross-sections continues to be of great interest. Numerous organic fluorophores with high TPF have been reported in literature, but their biological applications have been limited by their hydrophobicity and cellular toxicity. Here, we report the fabrication of fluorescent polymer nanoparticles (NPs) and their potential application as biomarker. These, NPs were fabricated using �mini-emulsion� technique. Strong TPF was observed from these polymeric NPs using femto-second laser pulses at 800 nm. Interestingly, these NPs were found to be non-toxic in a dose and time dependent manner in cell viability (MTT) studies. TPF microscopy imaging confirmed cellular uptake and two-photon activity of NPs in THP-1 cells. Diagnostic and theranostics applications of these novel NPs are been further explored.

High contrast two-photon fluorescence in vitro imaging using novel fluorescent polymeric nanoparticles

244th National Meeting (2012)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.