Simple and easily accessible methods to fabricate versatile multi-protein arrays with control not only over the specificity of the immobilization but also over the orientation of each of the immobilized proteins are in great demand in nanotechnology and life sciences.Reactive and fluorescent reporter monolayers, i.e. monolayers that have some reactive moiety at the head-group that allows simultaneous anchoring and visualization of the product of immobilization, are extremely useful for (bio)molecular (nano)fabrication.The current presentation focuses on a new strategy for the simultaneous detection and immobilization of thiols. An orthogonally modified coumarin has been used to fabricate a fluorogenic reactive monolayer platform on glass, for the covalent and selective immobilization of thiols. The platform properties in terms of reactivity, selectivity, sensitivity and co-localization towards thiols have been assessed. Adopting multifunctional orthogonal covalent and non-covalent immobilization strategies we demonstrate the direct oriented ligation of proteins onto the self-reporting fluorogenic reactive monolayer.

Thiol-reactive self-reporting surface as template for biomolecules

245th National Meeting (2013)

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