Near-Infrared (NIR) absorbing and fluorescent carbocyanine dyes are increasingly used as reporting labels, probes, imaging agents or markers in analytical, biological and medical field.  The far red and the NIR region (600-1100 nm) have clear advantages for the spectroscopist due to the inherently lower background.  This presentation focuses on analytical and bioanalytical applications that benefit from properties of NIR carbocyanines.  Several NIR dyes were prepared to determine the role of the hydrophobicity and charge of NIR dyes in binding to proteins, mostly serum albumins.  We synthesized NIR carbocyanine homologs containing the same chromophore but substituents of varying hydrophobicity and used them as probes to characterize binding sites.  Carbocyanines can also be used for reporting on enzyme activity as it is shown for the detection of the alkenesulfonate monooxygenase enzyme.  In addition this presentation will report examples of silica nanoparticles containing carbocyanines for applications requiring bright fluorescent labels.

NIR fluorescent carbocyanines: Analytical and bioanalytical applications

245th National Meeting (2013)

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