Tuberculosis (TB) is the world's second deadliest infectious disease after HIV/AIDS. The World Health Organization has called for the development of a robust, reliable, and accurate detection method that does not suffer from the poor accuracy of current serological-based techniques. We are working to develop a highly accurate, field-deployable platform with limited cross reactivity and low limits of detection that meets these critical needs. Our platform consists of a surface-enhanced Raman scattering-based sandwich immunoassay for the multiplexed detection of three different TB antigens: lipoarabinomannan, alpha-crystalline protein, and recombinant protein Rv0363. In our assay, an antigen is first selectively bound to a capture substrate and then tagged with an extrinsic Raman label, which consists of a gold nanoparticle modified with a Raman reporter molecule and coated with tracer antibodies. Research results to date for the multiplexed detection of these three TB markers in buffered medium and human serum will be discussed.

Multiplexed detection of <i>Mycobacterium tuberculosis</i> antigens using a surface-enhanced Raman scattering immunoassay platform

245th National Meeting (2013)

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Multiplexed detection of Mycobacterium tuberculosis antigens using a surface-enhanced Raman scattering immunoassay platform