This presentation will describe our research focused on the development of an ultrasensitive detection method for Luteinizing Hormone (LH) via a surface-enhanced Raman scattering (SERS)-based immunoassay. LH plays a key role in the development and function of the male and female reproductive systems. LH deficiency is associated with several pediatric disorders such as pituitary dysfunction and Kallmann syndrome. Early detection of these disorders can lead to more effective treatment. The current chemiluminescent assay has a limit of detection (LOD) of 4.7 pg/mL, which is insufficient for pediatric testing. Our SERS-based immunoassay promises to address this shortfall through the realization of an easy-to-use, ultrasensitive, selective, and rapid detection platform for LH. This assay involves the selective binding of LH to an antibody-functionalized capture substrate and subsequent labeling with an extrinsic Raman label (ERL). The ERL is composed of gold nanoparticles modified with both a Raman reporter molecule and a tracer antibody.

Detection of Luteinizing Hormone using a surface-enhanced Raman scattering-based immunoassay

245th National Meeting (2013)

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