We report the fabrication of laponite (LAP)-doped poly(lactic-co-glycolic acid) (PLGA) nanofibers for osteogentic differentiation of human mesenchymal stem cells (hMSCs). In this study, LAP-doped PLGA nanofibers were formed via simply electrospinning the mixture solution of PLGA and LAP. We show that the incorporation of LAP does not significantly change the uniform PLGA fiber morphology, instead significantly improves the mechanical durability of the nanofibers. The surface hydrophilicity and protein adsorption capacity of the composite nanofibers slightly increase after doping with LAP. Cytocompatibility results reveal that the incorporated LAP is beneficial to promote the cell adhesion and proliferation likely due to the improved surface hydrophilicity and protein adsorption capability of the fibers. Finally, we demonstrate that the doped LAP within the PLGA nanofibers is able to induce the osteoblast differentiation of hMSCs in complete medium without any inducing factors . The fabricated LAP-doped PLGA nanofibers may find many various tissue engineering applications.

Osteogenic differentiation of human mesenchymal stem cells onto electrospun laponite-doped poly(lactic-co-glycolic acid) nanofibers

245th National Meeting (2013)

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