Most of studies using nanometer-sized metal particles have focused on biological safety and potential hazards. However, we previously reported that platinum nanoparticles (Pt NPs) could scavenge reactive oxygen species (ROS) such as superoxide anion radical and hydroxyl radical, demonstrating intracellular ROS-scavenging activity of Pt NPs using cultured animal cells. In this study, we analyzed intracellular ROS-scavenging activity of Pt NPs in more details. Pt NPs did not show cytotoxicity on rat myoblast cells below 10 ppm. Pt NPs scavenged intracellular H2O2 dose-dependently. In addition, Pt NPs increased the amount of intracellular glutathione in the range of 0.01-1 ppm. Pt NPs also upregulated antioxidant-related gene expressions, suggesting that Pt NPs uptaken into cells regulate redox signaling pathways because Pt NPs scavenged intracellular ROS significantly even in a concentration as low as 0.01 ppm. The mechanism of action of Pt NPs will be discussed

Analysis of mechanism of intracellular reactive oxygen species-scavenging activity by platinum nanoparticles

245th National Meeting (2013)

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