As an extension of our work on salt tolerant ligands for membrane adsorber applications (1), we recently reported on our development of a guanidinium-functionalized AEX membrane adsorber that exhibits high protein binding capacities as well as excellent tolerance to high ionic strength (2).  This presentation will describe our journey from initial ligand candidate screening and identification, through membrane selection, to development and optimization of membrane functionalization methods with an eye towards the development of a practical large-scale manufacturing process.  The effects of ligand structure and of the nature of the microenvironment surrounding the ligand following membrane surface modification will be specifically addressed.  Optimized membrane absorbers display dynamic binding capacities for bovine serum albumin (BSA) of greater than 100 mg protein/mL of membrane volume, LRV's for virus of  > 6 at 150 mM NaCl, and efficient removal of host cell proteins and DNA in multivalent buffers.1.      �Salt tolerant membrane adsorbers for robust impurity clearance� William T. Riordan, Steven M. Heilmann, Kurt Brorson, Kannan Seshadri, Mark R Etzel, Biotechnol. Prog., 2009, 25, 1695-1702.2.      �Salt tolerant ligands and polymers for membrane absorber applications� Catherine Bothof, Jerald K. Rasmussen, Kannan Seshadri, Amitava Kundu, 241st ACS National Meeting, 2011, Abstract # BIOT 99.

Development of high performance anion-exchange membrane adsorbers

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.