Mitochondria, double-membrane-bound organelles are vital to the eukaryotic cell and contain their own DNA (mtDNA). Mitochondria is responsible for cellular respiration, the process of converting glucose into ATP. Antiviral ribonucleosides (AVRN), drugs used to help treat viruses cause toxicity due to its effect on mitochondrial transcription. Tissue culture and Northern blot were used to analyze effects of 2'-C-methyladenosine on mitochondrial transcription. Mitochondrial transcription was inhibited by EtBr and recovered in the presence or absence of 2'-C-me-A. AVRN's (such as 2'-C-me-A) are utilized by mitochondrial polymerase which causes toxicity, hence the lack of survival past all three phases of clinical trials.

Characterization of mitochondrial transcription by Northern blot

245th National Meeting (2013)

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