Nonfunctional ribosomal RNA decay (NRD) is a degradation pathway in Saccharomyces cerevisiae that targets and eliminates structurally intact but functionally defective rRNAs.  Two separate NRD pathways for 18S and 25S rRNA have been identified.  18S NRD requires translation elongation and involves the termination factor-like proteins Hbs1p and Dom34p, and the mRNA decay factors Ski7p and Xrn1p.  25S NRD, however, is not dependent on translation elongation and requires the ubiquitin E3 ligase component Rtt101p and its associated protein Mms1p.  Currently, we are using DNA microarray analysis to find differentially expressed genes during 18S and 25S NRD activation in S. cerevisiae.  We hope that discovering additional factors involved in these pathways will give us insight into the mechanisms of NRD, but also their relationship to other cellular RNA degradation pathways.

Microarray analysis of nonfunctional ribosomal RNA decay in <i>Saccharomyces cerevisiae</i>

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Microarray analysis of nonfunctional ribosomal RNA decay in Saccharomyces cerevisiae