Parahydrogen Induced Polarization can offer >20,000 fold increase in magnetic resonance signal under the right conditions. Diethyl 1-13C succinate is generated through the hydrogenation of diethyl 1-13C fumarate and hyperpolarization increases the 13C signal by 5000 fold. Previously using 13C magnetic resonance spectroscopy, metabolism of diethyl succinate in the Tricarboxylic Acid Cycle was observed in normal animals 5 s after injection. The biodistribution of the compound can be observed using 13C imaging. We have furthered our work by injecting hyperpolarized diethyl succinate via tail vein injection into BALB/c mice bearing a breast (4T1), renal tumor (RENCA), or lymphoma A20 tumor. We see more pronounced uptake in RENCA tumors versus A20 or 4T1 tumors.  The distribution of the hyperpolarized molecule reveals the regions of tumor heterogeneity.  Real time magnetic resonance spectroscopy and chemical shift imaging of hyperpolarized metabolites in vivo reveals the differences in metabolism in three different cancers.

Comparative metabolic fingerprinting employing hyperpolarized  diethylsuccinate in three cancer models in vivo

245th National Meeting (2013)

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