Nicotinamide is one of the two principal forms of vitamin B3, and is both a food nutrient and a drug. Molecular imprinted technology could be an approach to a rapid and low cost analytical method for this compound. During the polymer preparation, experimental variables have an impact on the molecular recognition and the structure of polymer. In this study, the effect of cross linker, its molar ratio to monomer and nicotinamide concentration was evaluated. The EGDMA crosslinked polymer at 1:4 molar ratio (monomer to cross-linker) gave the best specificity, while a similar value was obtained with the 1:2 TRIM crosslinked polymer. The most uniform microsphere structure was obtained with the EGDMA polymer whereas TRIM gave more aggregates. It was also found that increasing nicotinamide concentration during the polymerisation seems to ameliorate the binding capacity. Selectivity studies showed that the recognition of nicotinamide on MIP was due in part to the amide group and its position on the ring.

Optimization and characterization of molecularly imprinted polymers used for nicotinamide analysis

245th National Meeting (2013)

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