Rocky seashores are ecosystems with multiple life forms.  These organisms have an impact in our daily life  Their ecosystems have been threatened by contaminants that serve as an external selective pressure that can induce stress in these organisms. Our project explores the relationship between the expression of heat shock proteins of Nerita peloronta  and the zonation and water quality at Surfer's Beach, Aguadilla in Puerto Rico. It will explore if HSP levels of Nerita  can be use as a biomarker for environmental stress.  The Nerita peloronta  zonation will be correlated with physicochemical parameters.  Horizontal and vertical zonation will be examined, establishing two transepts and 10 quadrants per transepts. Heat shock protein will be isolated from total snail muscle proteins preparations, resolved in a Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis (SDS-PAGE). Western Blot analysis will be used to identify and quantify the protein level.

Heat shock proteins in Nerita peloronta as indicator of environmental stress at Surfer's Beach, Aguadilla, Puerto Rico

245th National Meeting (2013)

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