Aflatoxin producing members of Aspergillus flavus are universal and widespread in nature. Aflatoxins are toxic to humans and animals, and are one of the most carcinogenic of all natural compounds. Headspace solid phase microextraction (HS-SPME) combined with GCMS was used to collect and quantify volatile organic compounds (VOCs) emitted from A. flavus. Three different fibers were compared using 30 standard VOCs. Results indicate that fibers coated with PDMSCAR have the best efficiency for extracting alcohols, ketones and hydrocarbons produced by A.flavus at low concentrations. The volatile metabolites and the quantity of aflatoxins were monitored in day 6, 10, 14, 20 and 24. The relative compositions were compared among toxigenic and non-toxigenic fungi. The correlation between concentration levels of aflatoxins and volatile metabolites were used to identify volatile fingerprints produced by toxigenic A. flavus. HS-SPME combined with GCMS is a good approach for rapid discriminating between toxigenic and non-toxigenic isolates of A. flavus.

Analysis of volatile fingerprints for discriminating toxigenic and non-toxigenic <i>Aspergillus flavus</i>

245th National Meeting (2013)

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Analysis of volatile fingerprints for discriminating toxigenic and non-toxigenic Aspergillus flavus