The purpose of this project is to study the cisplatin-induced apoptosis mechanism in HeLa cells. Apoptosis or programmed cell death occurs when molecular lesions are formed in genomic DNA. By using small-interfering RNA (siRNA), the oncogene c-Abl will be knocked down. c-Abl encodes a cytoplasmic and nuclear protein tyrosine kinase involved early in the apoptotic pathway. A colorimetric assay kit will be used to measure the amount of caspase-3 present in cells (indicative of apoptosis) before and after knockdown of c-Abl. The results of this research will determine if c-Abl is involved in cisplatin mediated apoptosis in a cervical cancer cell line and may reveal potential drug resistance mechanisms.

Cisplatin-induced apoptosis in HeLa cells to determine potential mechanisms for drug resistance

245th National Meeting (2013)

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