Oxyvita, a zero-linked polymeric bovine hemoglobin is being investigated as a safer hemoglobin-based oxygen carrier (HBOC) due to its homogenous molecular size and structural stability. For oxygen transport and delivery to be effective, the heme-iron has to stay in the reduced form. Once the iron is oxidized, it is no longer able to carry oxygen. To determine the tendency of the Oxyvita Hb to oxidize, its reduction potentials (E�RED) were determined. Using Spectroelectrochemistry (SEC) and Nernst Plot analysis, Oxyvita Hb reduction potentials were investigated and compared to reduction potentials of other hemoglobins. The average reduction potentials for Oxyvita hemoglobin were -112.4�5.6 mV and -166.35�12.6 mV for wavelengths 406 nm and 43 0nm, respectively. These values indicate low resistance to oxidation. However, due to carbon monoxide interference, more trials are needed before any generalizations can be made.

Spectroelectrochemical characterization of the blood substitute, oxyvita

245th National Meeting (2013)

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