CDP-choline, also known as cytidine 5'-diphosphocholine or citicoline, is the active form of choline that is important for normal membrane function, acetylcholine synthesis, lipid transport, and methyl metabolism. CDP-choline supplements are available in the market to promote brain function. Once absorbed, CDP-choline is widely distributed throughout the body, crosses the blood-brain barrier and reaches the central nervous system, where it is incorporated into the membrane and microsomal phospholipid fraction. Despite its importance, only a limited number of assays are available in literature and have not been extensively applied to biological samples due to undesirable sensitivity and selectivity. In this study, a new liquid chromatography-selective reaction monitoring-mass spectrometry (LC/SRM/MS)-based assay is described for quantification of CDP-choline. The assay is evaluated by parameters such as precision, accuracy, linear dynamic range, limit of detection and limit of quantification. The concentrations of CDP-choline in various samples such as tissues, plasma and urine are determined.

Quantification of CDP-choline in tissues and body fluids using LC/SRM/MS and stable isotope dilution

245th National Meeting (2013)

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