The slime mold Stemonitis flavogenita was studied to determine the gene sequence for actin. Actin, a cytoskeletal protein, is highly conserved among eukaryotes; however, the actin sequence for this species is unknown. DNA and RNA were isolated from the aphanoplasmodial and coralloid stages of this organism. Known actin gene sequences were aligned and compared in order to determine the best primer sequences for PCR. After preparing cDNA samples from the RNA isolations through reverse transcription, the DNA and cDNA samples were amplified using PCR under various conditions. The amplified samples were analyzed using agarose gel electrophoresis. It was determined that samples that were amplified at temperatures near 37�C produced the largest bands, although they were not the strongest bands. The target DNA size is 1130 bases, and a couple of samples are larger than this. Additional isolations will be performed and analyzed and the results will be presented.

Isolation and determination of the DNA gene sequence of actin for the slime mold Stemonitis flavogenita

245th National Meeting (2013)

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