Genome integrity is essential for the survival of the cells; therefore the ability to respond to DNA damage is critical. Breast cancer-1 (BRCA1) participates in the DNA damage response and co-localizes with Abraxas and BRCC36, as it forms a multi-protein BRCA1 A-complex. The mechanism by which BRCA1 A-complex proteins functions remains elusive. Abraxas serves as an adapter protein in the complex. BRCC36 contains deubiquitination activity and has recently been observed to multimerize with itself. To further understand the role of BRCA1 A-complex proteins, we aim to dissect these genes functions through the generation of multiple mutants and their expression in cells for functional analysis. Abraxas cDNA mutants were able to detect transient expression in mouse embryonic fibroblasts cells. Dissected BRCC36 clones show that the multimerization domain is located between the MPN domain and Coiled-Coil domain. Further analysis will be performed to comprehend the organization and function of the complex.

Functional dissection of BRCA1 A complex proteins, Abraxas, and BRCC36

245th National Meeting (2013)

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