Replicative DNA polymerases have a role in the control of DNA synthesis and are potential targets for stopping unregulated cell growth, such as cancer.  DNA polymerase alpha, which assembles RNA primers and initiates replicative synthesis, represents a model enzyme in the study of this control.  This enzyme is composed of a priming subunit, a catalytic subunit, and two structural subunits.  Several primers developed from known enzyme gene sequences have been used to identify an expressed sequence tag (EST) in the catalytic subunit of the enzyme in embryonic chicken brain.  The sequence of the EST matches a published chicken genomic DNA sequence.  This EST will be used in real time PCR to help quantitate DNA polymerase alpha catalytic subunit mRNA under various growth conditions.

Expressed sequence tag for DNA polymerase alpha

245th National Meeting (2013)

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