The cohesin protein complex is vital for holding sister chromatids together during cell division. In Arabidopsis thaliana, a-kleisin proteins are the connectors of two structural maintenance proteins in the cohesin complex. The four a-kleisin proteins found to be vital to sister chromatid cohesion in Arabidopsis thaliana are SYN1, SYN2, SYN3, and SYN4. The behavior of SYN3 was examined in previous research, and it was found not to aggregate around chromosomes in the way SYN1, SYN2, and SYN4 do; instead, its predominant localization in the nucleolus lead to the hypothesis that SYN3 is involved in transcriptional regulation. By employing a yeast two-hybrid assay to screen for SYN3-interacting proteins and using subsequent co-immunoprecipitation and western blotting to confirm the potential interactions, SYN3's putative role in transcriptional regulation was investigated. SYN3 was ultimately shown to interact with a number of known transcription factors, suggesting a regulatory function in gene expression for the protein.

Identification of proteins that interact with the arabidopsis SYN3 cohesin protein

245th National Meeting (2013)

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