Cohesins are critical for holding a pair of sister chromatids together before segregation during cell division, as well as a variety of other processes. The core cohesin complex consists of 4 proteins: SMC1, SMC3, SCC3, and an ?-kleisin. In Arabidopsis thaliana, the 4 ?-kleisin proteins are SYN1, SYN2, SYN3, and SYN4. SYN3 was found to be enriched in the nucleolus and is crucial for gametophyte growth. Recent studies have also shown that SYN3 cohesins in animal cells may have regulatory roles in transcription. Utilizing the Matchmaker Gold Yeast Two-Hybrid Assay, SYN3 was tested against a protein library to screen for potential interacting partners. Over 60 proteins were determined to be candidates for SYN3's interactome. Among these partners were a number of chloroplast-localized proteins including FTSZ-1: the Arabidopsis homolog of the bacterial Z-ring protein. These interactions are interesting because SYN3 has never been shown to have a role in the chloroplast.

Identifying the interacting partner protiens of cohesin SYN3

245th National Meeting (2013)

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