Regulation of reactive oxygen species is an essential function in cellular health.  Disruption in cellular redox regulation is a hallmark of atherosclerosis, cancer, neurodegenerative disease, and aging.  Therefore, it is critical to monitor the proteins that maintain redox homeostasis.  One such protein residue implicated in cellular redox maintenance is cysteine sulfenic acid.  Yet, despite its putative role as a messenger in redox regulation, very few methods exist for its real-time analysis. Of the chemical probes used to detect cysteine sulfenic acid, dimedone is the most widely used reagent. In this report, we disclose an examination of dimedone selectivity and introduce alternative probes for the bioorthogonal detection of cysteine sulfenic acid. Applications in the analysis of oxidative stress are discussed.

Chemical methods for the detection of cysteine sulfenic acid in biological systems

245th National Meeting (2013)

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