Apoptosis is a genetically controlled process by which a cell dies naturally. It is initiated through activation of effector Caspases 3/7. Morinda citrifolia is used as a dietetic supplement because of its antioxidant, anti-inflammatory and hepatoprotective properties. Noni shell extracts, aqueous and organic, and pulp extract were prepared. CHO cells were cultured. After treatment with the plant's extracts for a 96-hour period, a colorimetric substrate was applied and incubated for four hours at 37�C. Absorbance measurements were determined. Preliminary results were 0.00050 pmol/�L for pulp, 0.00480 pmol/�L aqueous extract, and 0.0100 pmol/�L for organic extract. Results suggest organic extract from Noni shell had and inductive effect on Caspase 3 activity. Other assays will be conducted to verify these results and establish specific conclusions.

Detection of caspase 3 activity in Chinese hamster ovarian cells after treatmeant with Morinda citrifolia (Noni) extract

245th National Meeting (2013)

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