�Polymer brushes� have been the subject of many investigations over the last years, with their potential to be used as versatile, stimuli-responsive surface coatings. Polymer chains grafted to a planar or curved substrate exhibit stimuli-responsive behavior due to conformational changes determined by the chemical nature of the polymer. Furthermore, these coatings offer the possibility to introduce additional functionality, for example by immobilization of nanoparticles or biomolecules, either via physical adsorption or chemical binding. We report on the immobilization of the model enzyme glucose-oxidase (GOx) to pH-responsive poly(acrylic acid) (PAA) guiselin brushes. Carbodiimide chemistry was used to covalently couple the enzyme to the polymer. Insitu investigations of Quartz-Crystal-Microbalance with Dissipation mode and Attenuated Total Reflection- Fourier Transform Infrared Spectroscopy have been used to confirm the successful bonding of the amine groups of the enzyme to the carboxylic groups of the polymer. The catalytic activity of the enzyme was confirmed using a colorimetric assay, proving the coupling of GOx to PAA in a non-inhibiting manner.

Biocatalytic surface coatings by covalent immobilization of enzymes in polymer brushes

245th National Meeting (2013)

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