Bacterial sepsis is a serious clinical condition that can lead to multiple organ dysfunction and death despite timely treatment with antibiotics and fluid resuscitation. Gram-negative bacteria are a common causative pathogens in bacterial sepsis. To develop an approach to clearing Gram-negative bacteria from the bloodstream, we developed magnetic nanoparticles (MNPs) modified with bis-Zn-DPA, a synthetic ligand that binds to Gram-negative bacteria. We used a magnetic microfluidic device to effectively remove MNPs bound to E. coli cells (a gram-negative bacteria) from bovine whole blood. When E. coli contaminated blood (5.0 x 106 CFU/mL) mixed with MNPs was circulated through multiple magnetic microfluidic devices at a maximum rate of 60 mL/h, we observed greater than 95 % clearance of E. coli. We demonstrate that bis-Zn-DPA functionalized MNPs selectively associate with E. coli, and facilitate its clearance. This system offers an efficient and cost-effective method for clearing pathogens from whole blood.

Zn-DPA functionalized magnetic nanoparticles for selective microfluidic filtration of pathogens from blood

245th National Meeting (2013)

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