Mitochondrial oxidative stress is implicated in aging and many disorders including neurodegenerative diseases, diabetes, stroke, ischemia/reperfusion injury, age-related macular degeneration (AMD) and cancer. Recently, we have developed two new mitochondria-targeting fluorescent probes, MitoProbes I/II , which can specifically localize in mitochondria and can be used for in vivo and in vitro mitochondrial oxidative stress detection. In this presentation, we will report the design and synthesis of MitoProbes, as well as demonstrate their application for the detection of mitochondrial oxidative stress in living human retinal pigment epithelial cells, in ischemia/reperfusion (I/R) injured rats and in amyotrophic lateral sclerosis (ALS) afflicted mice. Through the use of MitoProbes, imaging mitochondrial redox status can be clinically implemented utilizing cryogenic biopsy specimens and will be proven useful for drug development and for clinical diagnosis of oxidative stress-mediated diseases.

Mitochondria-targeted fluorescent probes for detection of mitochondrial oxidative stress

245th National Meeting (2013)

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