Insitu chemical modification and mass spectrometric mapping (IC-MSM) is a promising tool for characterizing the orientation and localized structural shifts (collectively termed configuration) in adsorbed proteins. However, the current application of this technique is limited to quantifying the labeling profile of a single population of target amino acid as reactivity rate of labeling is specific to a given amino acid and the type of chemical label used. As a result, a direct comparison of the labeling profile of amino acids modified with different chemical labels may not be feasible and could limit a comprehensive understanding on the configuration of adsorbed protein using IC-MSM. In our study we have developed a quantification technique which would allow a direct comparison of the reactivity rate of multiple amino acids which could subsequently aid significantly improving the capabilities of IC-MSM as a tool for characterizing the configurational shifts in adsorbed protein on different surfaces.

Determination of adsorbed protein configuration on material surfaces by in situ chemical modification and mass spectrometric mapping

245th National Meeting (2013)

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