17�-estradiol (abbreviated E2) is a common hormone and endocrine-disrupting compound. Conventional detection of E2 is typically performed using gas or liquid chromatography coupled with high-resolution mass spectroscopy; methods associated with this approach are time consuming, require a high level of expertise, and are expensive.  To overcome these challenges, we are immobilizing a DNA aptamer developed for E2 on an interdigitated array (IDA) electrode for fast, efficient, low cost detection of E2.  The IDA electrode offers the advantage of enhanced sensitivity over conventional electrodes, because they contain multiple pairs of generator and collector electrodes which enhance redox couple cycling necessary for electrochemical detection.  A primary challenge for E2 detection is interference from natural organic matter (NOM). Cyclic voltammetry, square wave voltammetry, and electrical impedance spectroscopy are being used to evaluate the sensitivity of E2 detection using the DNA aptamer � IDA electrode system, and the mechanisms of NOM interference with detection.

Detection of 17β-estradiol in drinking water sources using DNA aptamer-based electrochemical detection

245th National Meeting (2013)

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.