SERS is used as a label-free analytical technique to quantify RNA hybridization to oligonucleotide capture probes immobilized on silver nanorods (AgNR).  Previously, we reported that SERS is highly sensitive, showing a change in signal upon hybridization of target RNA to complimentary probes and LS regression allows for nucleotides to be quantified.  Improvements in the LS model are anticipated with a better understanding of the maximum hybridization efficiency.  To this end, fluorescently labeled probes are employed to determine the surface concentration of immobilized probes on the AgNR substrate.  Subsequently, FAM-labeled target RNA is hybridized to the capture probe to determine the maximum hybridization efficiency.  The effects of a 3' spacer on the capture probe and mercaptohexanol on hybridization efficiency are investigated.  These results will lead to an improved LS model for more accurate quantification of target RNA and an optimized capture probe layer for optimum sensitivity.

Investigation and optimization of DNA hybrid of AgNR

245th National Meeting (2013)

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