Dynamic IEF is a new method related to capillary isoelectric focusing that can provide good resolution and separation capabilities by controlling the shape of the electric field using additional high voltage power supplies. Both the location and width of the focused protein bands can be controlled by manipulation of the electric field.Current research focuses on demonstrating and improving the performance characteristics of the system. Hemoglobin variants A, F, S, C have been separated using dynamic IEF to illustrate that this method has the same separation capabilities as capillary IEF. In order to establish what variant has been separated, the four variant standards have been digested and analyzed using gel electrophoresis and ESI-MS.

Validating dynamic IEF using hemoglobin variants

245th National Meeting (2013)

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