1H nuclear magnetic resonance (1H-NMR) was used to investigate the metabolism of arabinose and xylose in Clostridium acetobutylicium under anaerobic condition.  The fermentation products were separated by HPLC and quantified with exterior standards.  The results show that in arabinose fermentation in C. acetobutylicium, more acetate was produced than butyrate.  On the other hand, in xylose fermentation under the same conditions, a slightly more butyrate was produced than acetate.  Isotope labeled L-[1-13C]arabinose and D-[1-13C ]xylose were introduced to study the metabolism of the fermentation.  The results show that in arabinose, much more isotope labeled carbons is on the C-2 position of acetate (48% in arabinose over 38% in xylose).  The results provide evidences that arabinose is metabolized via a phosphoketolase pathway in C. acetobutylicium.

<sup>1</sup>H nuclear magnetic resonance studies of arabinose and xylose metabolism in Clostridium acetobutylicium

245th National Meeting (2013)

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1H nuclear magnetic resonance studies of arabinose and xylose metabolism in Clostridium acetobutylicium

¹H nuclear magnetic resonance studies of arabinose and xylose metabolism in Clostridium acetobutylicium