Cell-free biosystems for biomanufacturing (CFBB) are the implementation of complicated reactions that microbes and chemical catalysts cannot do through the in vitro assembly of numerous (stable) enzymes originated from different sources and/or (biomimetic) cofactors [1]. CFBB features numerous advantages: high product yield, fast reaction rate, easy access and control for open systems, tolerance of toxic compounds, broad reaction conditions, and so on. In this talk, I will present key advances in three major applications: (i) the theoretical yield hydrogen production from sugars (e.g., pentoses and hexoses), (ii) conversion of cellulose to starch, and (iii) super-energy sugar-powered density enzymatic fuel cells. CFBB would become a high-yield and low-cost biomanufacturing platform and lead to a biotechnology paradigm shift, especially in the sustainability revolution associated with energy-water-food nexus [2]  .References 1. Zhang Y-HP, Sun J-B, Zhong J-J: Biofuel production by in vitro synthetic pathway transformation  . Curr. Opin. Biotechnol. 2010, 21 :663-669.2. Zhang Y-HP, Huang W-D: Constructing the electricity-carbohydrate-hydrogen cycle for a sustainability revolution  . Trends Biotechnol. 2012, 30 :301-306.

Biomanufacturing paradigm shift: From living microorganisms to cell-free biosystems

245th National Meeting (2013)

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