Bacterial cellulose (BC) had been synthesized from Gluconacetobacter xylius (ATCC-10245) strain using two different carbon source, glusoce and mannitol in Hestrin and Schramm (HS) medium. A thick gelatinous film with high water retaining capacity had been produced from the purification of BC after the cultivation period. The morphological changes were observed by FE-SEM and FTIR analysis after drying the sample in vacuum at 80�C till constant weight. A three dimensional network of fibrils with porous structure similar to extracellular matrix had been confirmed by the FE-SEM having an average fiber diameter range of 82�18 nm. According to FTIR results, the absorbance peak at 750 and 710 cm-1 indicated the presence of cellulose Ia content indicating high crystallinity index compared to plant cellulose which is rich in I� content. The directional porous structure with high crystallinity index would make this material suitable for articular tissue engineering and other biomedical applications.

Development of novel cellulosic-based scaffolds for articular cartilage tissue engineering by critical directional freeze drying

245th National Meeting (2013)

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