A series of antioxidant profiling experiments for undergraduate biochemistry laboratory is described. The antioxidant status is estimated for several species of unicellular algae by analysis of cell lysates for peroxidase activity, superoxide scavenging, and total antioxidant capacity. This series may also be used to compare antioxidant activity in a single species under variable growth conditions of salinity, available nitrogen, and temperature. These experiments are designed to exemplify concepts in redox biochemistry, to familiarize students with enzyme analysis, and to introduce the theory and practice of luminescence techniques in biochemistry.

Antioxidant profiling experiment for undergraduate biochemistry

245th National Meeting (2013)

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