The enzymatic hydrolysis of cotton raw cellulose (RC) samples, mercerized RC samples (M-C), freeze-dried RC (RC-FD) samples, microcrystalline cellulose Avicel, bacterial cellulose (BC), raw sisal pulp (RS) and mercerized sisal pulp (S-M) was performed at cellulose-to-cellulase mass ratios ranging from 1000:1 to 10:1. The most efficient hydrolysis ranged from 70 % and 85% for loading of 100:1 and 10:1, regardless the cellulosic sample. The hydrolysis efficiency decreased when the loading was greater than 400:1. The water sorption ability could be directly correlated with the hydrolysis yield, except for RC-FD and BC samples, which presented the lowest values. In the former, freeze-drying has led to pore collapse, with concomitant reduction of the amount of adsorbed water. The presence of nanofibrils on the surface of BC improved the accessibility, indicating that analysis by complementary techniques should be performed in order to predict the accessibility of cellulosic samples to cellulase.

Water sorption value and enzyme loading as important parameters for the enzymatic hydrolysis of cellulose

245th National Meeting (2013)

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