Pharaonis phoborhodopsin (ppR) is a negative phototaxis receptor of Natronomonus pharaonis and forms a 2:2 complex with the cognate transducer (pHtrII), which transmits the photosignal into cytoplasm. Light absorption of ppR initiates trans-cisphoto-isomerization of the retinal chromophore followed by cyclic chemical reaction consisting of several intermediates (K, L, M and O). We have successfully trapped and observed the M intermediate by using newly developed photo-irradiated solid-state NMR system and a new photo-cycle will be presented.

Photo-induced structural changes of photoreceptor membrane proteins as revealed by in-situ photo-irradiated solid-state NMR

245th National Meeting (2013)

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